Development of 14 polymorphic microsatellite loci for Ficus tikoua (Moraceae)1

PREMISE OF THE STUDY Polymorphic microsatellite markers were developed to facilitate studies on the fine-scale population genetic structure of Ficus tikoua (Moraceae), a prostrate shrub known to have highly restricted gene flow. METHODS AND RESULTS Microsatellite primers were developed using the biotin-streptavidin capture method and scanned for polymorphism within 76 individuals sampled from three natural F. tikoua populations. Fourteen loci were shown to be polymorphic, with allele numbers ranging from three to 16. The observed and expected heterozygosity in the three populations ranged from 0 to 1 and from 0 to 0.87, respectively. Substantial divergence was found among the populations at some loci. All loci can be successfully amplified in at least eight other Ficus species, indicating good transferability within the genus. CONCLUSIONS The 14 microsatellite loci will be a helpful tool for assessing the fine-scale genetic structure of F. tikoua.